Explanation
H group of one amino acid and -NH2 group of another with loss of water. Option A (“Two amino groups”) is incorrect because it does not satisfy the chemical formula, reaction, periodic trend, numerical relation, oxidation state or definition required by the question. Option B (“Two carboxyl groups”) is incorrect because it does not satisfy the chemical formula, reaction, periodic trend, numerical relation, oxidation state or definition required by the question. Option D (“Two hydroxyl groups”) is incorrect because it does not satisfy the chemical formula, reaction, periodic trend, numerical relation, oxidation state or definition required by the question. Identify the governing formula, definition or reaction and apply it consistently. The answer field, correct index and explanation therefore all identify Amino group and carboxyl group. Peptide bond forms between -COOH group of one amino acid and -NH2 group of another with loss of water.